Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Abstract Pepper vein yellows viruses (PeVYV) are phloem-restricted in the genus Polerovirus , family Luteoviridae . Typical viral symptoms of PeVYV including interveinal yellowing leaves and upward leaf curling were observed pod pepper plants ( Capsicum frutescens ) growing Wenshan city, Yunnan province, China. The complete genome sequence a virus from sample these was determined by next-generation sequencing RT-PCR. Pod (PoPeVYV) (MT188667) has 6015 nucleotides, characteristic organization member In 5? half its (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) PeVYV-3 3 (KP326573) but diverges greatly 3?-part encoding P5, where it (91.7% tobacco distorting (TVDV, EF529624) suggesting recombinant origin. Recombination analysis predicted single recombination event affecting nucleotide positions 4126 5192 nt, with as major parent region 4126–5192 derived TVDV minor parent. A full-length clone constructed shown be infectious C. RT-PCR presence icosahedral particles.